EasyPro Universal Leukotriene E4(LTE4)ELISA Kit

Universal

Cysteinyl Leukotrienes E4

Competitive

2000pg/mL

9.41 pg/mL

31.25-2000 pg/mL

45min

serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Metabolic pathway;Infection immunity;Autoimmunity;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTE4 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for LTE4 are added to the micro ELISA plate wells. LTE4 in samples (or standards) competes with a fixed amount of LTE4 on the solid phase supporter for sites on the HRP linked detection antibody specific to LTE4. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve.