EasyPro Universal Cyclic Adenosine Monophosphate(cAMP)ELISA Kit

Universal

c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

Competitive

20000pg/mL

92.5 pg/mL

312.5-20000 pg/mL

45min

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Signal transduction;Metabolic pathway;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for cAMP are added to the micro ELISA plate wells. cAMP in samples (or standards) competes with a fixed amount of cAMP on the solid phase supporter for sites on the HRP linked detection antibody specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.