EasyPro Universal Carnosine(Car)ELISA Kit

Universal

β-Alanyl-L-Histidine

Competitive

900ng/mL

3.53 ng/mL

14.07-900 ng/mL

45min

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Car Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Car are added to the micro ELISA plate wells. Car in samples (or standards) competes with a fixed amount of Car on the solid phase supporter for sites on the HRP linked detection antibody specific to Car. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Car in the samples is then determined by comparing the OD of the samples to the standard curve.