EasyPro Universal 4-Hydroxynonenal(4-HNE)ELISA Kit

Universal

4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal

Competitive

40ng/mL

0.38 ng/mL

0.63-40 ng/mL

45min

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for 4-HNE are added to the micro ELISA plate wells. 4-HNE in samples (or standards) competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the HRP linked detection antibody specific to 4-HNE. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.