一步法大鼠血管紧张素Ⅰ(Ang-Ⅰ)酶联免疫吸附测定试剂盒

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快速检测系列ELISA试剂盒

货号

FY-ER2892S

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

检测时间

45min

价格(美元)

300 420

规格

48T 96T

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DataSheet

说明书 MSDS

MSDS

产品详情

英文名称:

EasyPro Rat Angiotensin Ⅰ(Ang-Ⅰ)ELISA Kit

种属:

Rat

别称:

Angiotensin-1

检测方法:

Competitive

标准品:

1000pg/mL

灵敏度:

9.38 pg/mL

检测范围:

15.63-1000pg/mL

反应时间:

45min

样品类型:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

研究领域:

Signal transduction;

测试原理:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅰ Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Ang-Ⅰ are added to the micro ELISA plate wells. Ang-Ⅰ in samples (or standards) competes with a fixed amount of Ang-Ⅰ on the solid phase supporter for sites on the HRP linked detection antibody specific to Ang-Ⅰ. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Ang-Ⅰ in the samples is then determined by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD
1000	0.3	0.301	0.301
500	0.4	0.406	0.403
250	0.594	0.603	0.599
125	0.855	0.862	0.859
62.5	1.15	1.153	1.152
31.25	1.545	1.562	1.554
15.63	1.859	1.86	1.86
0	2.523	2.555	2.539

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	93-107	100
EDTA plasma (n=8)	87-99	93
Cell culture media (n=8)	87-95	91

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	84-99	95-104	86-97
1:2	Average (%)	86	96	91
1:4	Range (%)	83-95	95-104	86-93
1:4	Average (%)	90	104	92
1:8	Range (%)	83-95	80-92	91-96
1:8	Average (%)	84	90	92
1:16	Range (%)	96-101	82-95	87-98
1:16	Average (%)	99	83	90

检测流程图