EasyPro Mouse D-Dimer(D2D)ELISA Kit

Mouse

D2D

Competitive

5000ng/mL

46.88 ng/mL

78.13-5000 ng/mL

45min

Plasma and other biological fluids；Sample Volume=50μL

Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with D2D Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for D2D are added to the micro ELISA plate wells. D2D in samples (or standards) competes with a fixed amount of D2D on the solid phase supporter for sites on the HRP linked detection antibody specific to D2D. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of D2D in the samples is then determined by comparing the OD of the samples to the standard curve.