EasyPro Mouse Beta Crosslaps(β-CTx)ELISA Kit

Mouse

b-CTx, bCTXI, bCTX-I, B-Cr, BCL, Type I Collagen C-Telopeptide-Related Fraction

Competitive

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Signal transduction;Hematology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-CTx Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for β-CTx are added to the micro ELISA plate wells. β-CTx in samples (or standards) competes with a fixed amount of β-CTx on the solid phase supporter for sites on the HRP linked detection antibody specific to β-CTx. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of β-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.