EasyPro Mouse Angiotensin 1-9(Ang1-9)ELISA Kit

Mouse

Angiotensin 1-9

P11859(25~33aa)

Competitive

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Signal transduction;Neuro science;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang1-9 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Ang1-9 are added to the micro ELISA plate wells. Ang1-9 in samples (or standards) competes with a fixed amount of Ang1-9 on the solid phase supporter for sites on the HRP linked detection antibody specific to Ang1-9. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Ang1-9 in the samples is then determined by comparing the OD of the samples to the standard curve.