EasyPro Human Superoxide Dismutase 1, Soluble(SOD1)ELISA Kit

Human

ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]

P00441

Competitive

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Signal transduction;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for SOD1 are added to the micro ELISA plate wells. SOD1 in samples (or standards) competes with a fixed amount of SOD1 on the solid phase supporter for sites on the HRP linked detection antibody specific to SOD1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.