EasyPro Human Delta Sleep Inducing Peptide α(δSIP-α)ELISA Kit

Human

δSIP-α

Competitive

8000pg/mL

75 pg/mL

125-8000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Enzyme & Kinase;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with δSIP-α Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for δSIP-α are added to the micro ELISA plate wells. δSIP-α in samples (or standards) competes with a fixed amount of δSIP-α on the solid phase supporter for sites on the HRP linked detection antibody specific to δSIP-α. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of δSIP-α in the samples is then determined by comparing the OD of the samples to the standard curve.