一步法人Apelin 13蛋白(AP13)酶联免疫吸附测定试剂盒

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快速检测系列ELISA试剂盒

货号

FY-EH2912S

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

检测时间

45min

价格(美元)

300 420

规格

48T 96T

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DataSheet

说明书 MSDS

MSDS

产品详情

英文名称:

EasyPro Human Apelin 13(AP13)ELISA Kit

种属:

Human

别称:

AP13

Uniport ID:

Q9ULZ1(65~77aa)

检测方法:

Competitive

标准品:

8000pg/mL

灵敏度:

75 pg/mL

检测范围:

125-8000pg/mL

反应时间:

45min

样品类型:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

研究领域:

Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

测试原理:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with AP13 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for AP13 are added to the micro ELISA plate wells. AP13 in samples (or standards) competes with a fixed amount of AP13 on the solid phase supporter for sites on the HRP linked detection antibody specific to AP13. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD
8000	0.204	0.205	0.205
4000	0.361	0.363	0.362
2000	0.571	0.58	0.576
1000	0.835	0.845	0.84
500	1.119	1.133	1.126
250	1.419	1.433	1.426
125	1.753	1.778	1.766
0	2.549	2.568	2.559

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	80-96	88
EDTA plasma (n=8)	80-92	86
Cell culture media (n=8)	80-95	86

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	92-104	85-92	80-96
1:2	Average (%)	92	87	92
1:4	Range (%)	87-96	82-96	89-102
1:4	Average (%)	91	94	97
1:8	Range (%)	89-101	87-103	85-97
1:8	Average (%)	94	91	94
1:16	Range (%)	82-90	95-102	80-97
1:16	Average (%)	83	98	85

检测流程图