快速检测系列ELISA试剂盒

一步法大鼠低氧诱导因子1α(HIF-1α)酶联免疫吸附测定试剂盒

货号

FY-ER2787S

样本类型

Tissue homogenate, Cell lysate;Sample Volume=50uL

检测时间

1.5H

价格(美元)

规格

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产品详情
英文名称:
EasyPro Rat Hypoxia Inducible Factor 1 Alpha(HIF-1α)ELISA Kit
种属:
Rat
别称:
HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
Uniport ID:
O35800
检测方法:
Sandwich
标准品:
500pg/mL
灵敏度:
4.69 pg/mL
检测范围:
7.82-500pg/mL
反应时间:
1.5H
样品类型:
Tissue homogenate, Cell lysate;Sample Volume=50uL
研究领域:
Enzyme & Kinase;
测试原理:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HIF-1α. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for HIF-1α are added to the micro ELISA plate wells. HIF-1α in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to HIF-1α. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1α. You can calculate the concentration of HIF-1α in the samples by comparing the OD of the samples to the standard curve.
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
500 2.498 2.536 2.517 2.453
250 1.642 1.672 1.657 1.593
125 0.976 0.992 0.984 0.92
62.5 0.457 0.457 0.457 0.393
31.25 0.248 0.251 0.25 0.186
15.63 0.15 0.151 0.151 0.087
7.82 0.098 0.099 0.099 0.035
0 0.063 0.064 0.064 0
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
样本类型 Range(%) Average Recovery(%)
Serum (n=8) 87-99 93
EDTA plasma (n=8) 80-93 85
Cell culture media (n=8) 85-97 91
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-104 85-92 90-99
Average (%) 104 91 97
1:4 Range (%) 93-102 85-92 95-103
Average (%) 101 90 101
1:8 Range (%) 82-97 80-96 86-92
Average (%) 91 81 86
1:16 Range (%) 92-101 93-105 91-102
Average (%) 95 94 98
检测流程图
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