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一步法小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫吸附测定试剂盒

货号

FY-EM2840S

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL

检测时间

1.5H

价格(美元)

规格

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产品详情
英文名称:
EasyPro Mouse Neutrophil Gelatinase Associated Lipocalin(NGAL)ELISA Kit
种属:
Mouse
别称:
LCN2, Lipocalin 2, Oncogene 24p3, MSFI
Uniport ID:
P11672
检测方法:
Sandwich
标准品:
2500pg/mL
灵敏度:
23.44 pg/mL
检测范围:
39.07-2500pg/mL
反应时间:
1.5H
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
研究领域:
Enzyme & Kinase
测试原理:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NGAL. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for NGAL are added to the micro ELISA plate wells. NGAL in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to NGAL. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NGAL. You can calculate the concentration of NGAL in the samples by comparing the OD of the samples to the standard curve.
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
2500 2.308 2.318 2.313 2.289
1250 1.514 1.531 1.523 1.499
625 0.883 0.884 0.884 0.86
312.5 0.4 0.401 0.401 0.377
156.25 0.221 0.221 0.221 0.197
78.13 0.121 0.123 0.122 0.098
39.07 0.066 0.067 0.067 0.043
0 0.024 0.024 0.024 0
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
样本类型 Range(%) Average Recovery(%)
Serum (n=8) 85-103 94
EDTA plasma (n=8) 95-105 100
Cell culture media (n=8) 93-101 97
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 89-101 93-101 93-102
Average (%) 101 97 94
1:4 Range (%) 87-98 97-103 81-96
Average (%) 90 97 92
1:8 Range (%) 92-104 91-99 93-106
Average (%) 99 92 93
1:16 Range (%) 82-96 90-98 82-95
Average (%) 83 96 89
检测流程图
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