EasyPro Human Endostatin(ES)ELISA Kit

Human

Sandwich

40ng/mL

0.38 ng/mL

0.63-40ng/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Metabolic pathway;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ES. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for ES are added to the micro ELISA plate wells. ES in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to ES. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ES. You can calculate the concentration of ES in the samples by comparing the OD of the samples to the standard curve.