Rat PGD2S(Prostaglandin D2 Synthase) ELISA Kit

Rat

PTGDS, L-PGDS, LPGDS, PDS, PGD2, PGDS, PGDS2, prostaglandin D2 synthase, Beta-trace protein, Cerebrin-28, Glutathione-independent PGD synthase

Sandwich

200ng/mL

1.88 ng/mL

3.13-200ng/mL

3.5H

serum, plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Infection immunity;Hematology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGD2S. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGD2S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PGD2S, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGD2S in the samples is then determined by comparing the OD of the samples to the standard curve.