Rat CMLC-1(Cardiac Myosin Light Chain 1) ELISA Kit

Rat

MYL3, CMH8, MLC1SB, MLC1V, VLC1, MLC-lV/sb, VLCl, myosin light chain 3, Cardiac myosin light chain 1, Ventricular myosin alkali light chain, Ventricular/slow twitch myosin alkali light chain, Myosin light chain 1 slow-twitch muscle B/ventricular isoform, CMLC1

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

serum, plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Infection immunity;Hematology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CMLC-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CMLC-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CMLC-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CMLC-1 in the samples is then determined by comparing the OD of the samples to the standard curve.