Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Rat

DIF, TNF-alpha, TNFA, TNFSF2

P16599

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

serum, plasma, Tissue homogenate and Other biological samples

Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNF-α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF-α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF-α in the samples is then determined by comparing the OD of the samples to the standard curve.