Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Rat

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

P16599

Sandwich

50pg/mL

0.15 pg/mL

0.78-50 pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNF-α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF-α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF-α in the samples is then determined by comparing the OD of the samples to the standard curve.