Rat REG(Regenerating Islet Derived Protein) ELISA Kit

Rat

ICRF, REG1, PSP, PTP, Islet cells regeneration factor, Lithostathine, Islet of Langerhans regenerating protein, Pancreatic stone protein, Pancreatic thread protein

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Tumor immunity;Cardiovascular biology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to REG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of REG in the samples is then determined by comparing the OD of the samples to the standard curve.