Rat β-Hex B(β-hexosaminidase B) ELISA Kit

Rat

HEXB, ENC-1AS, HEL-248, HEL-S-111, hexosaminidase subunit beta

Sandwich

50ng/mL

0.47 ng/mL

0.79-50ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to β-Hex B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to β-Hex B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain β-Hex B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of β-Hex B in the samples is then determined by comparing the OD of the samples to the standard curve.