Rat IL-2Rα/CD25(Interleukin-2 Receptor alpha chain) ELISA Kit

Rat

Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha, IL2-RA,TAC antigen,p55,CD25

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Metabolic pathway;Hepatology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-2Rα/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-2Rα/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-2Rα/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-2Rα/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.