Rat PRKAA1(Protein Kinase, AMP Activated Alpha 1) ELISA Kit

Rat

PRKAA1, AMPK, AMPKa1, Protein kinase, AMP-activated, alpha 1, protein kinase AMP-activated catalytic subunit alpha 1

P54645

Sandwich

200ng/mL

1.88 ng/mL

3.13-200ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Metabolic pathway;Nutrition metabolism;Dermatology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRKAA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PRKAA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PRKAA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PRKAA1 in the samples is then determined by comparing the OD of the samples to the standard curve.