Rat RANTES(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

Rat

CCL5, SCYA5, SISd, TCP228, D17S136E

P50231

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RANTES in the samples is then determined by comparing the OD of the samples to the standard curve.