Rat CENPI(Centromere Protein I) ELISA Kit

Rat

CENP-I, FSHPRH1, LRPR1, Mis6,CENPI,FSH primary response protein 1,Follicle-stimulating hormone primary response protein,Interphase centromere complex protein 19,Leucine-rich primary response protein 1,ICEN19

Sandwich

50ng/mL

0.47 ng/mL

0.79-50ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Developmental science;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CENPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CENPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CENPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CENPI in the samples is then determined by comparing the OD of the samples to the standard curve.