Rat IL-17A(Interleukin 17A) ELISA Kit

Rat

CTLA8,?CTLA-8,?CTLA8cytotoxic?T-lymphocyte-associated?serine?esterase?8,?Cytotoxic?T-lymphocyte-associated?antigen?8,?IL17,?IL-17,?IL17A,?IL-17A,?IL-17Acytotoxic?T-lymphocyte-associated?protein?8,?IL-17CTLA-8

Q61453

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Endocrinology;Neuro science;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-17A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-17A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-17A in the samples is then determined by comparing the OD of the samples to the standard curve.