Rat MRC1(Mannose Receptor C Type 1) ELISA Kit

Rat

CD26,C-type lectin domain family 13 member D,C-type lectin domain family 13 member D-like,Macrophage mannose receptor 1-like protein 1

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MRC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MRC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MRC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MRC1 in the samples is then determined by comparing the OD of the samples to the standard curve.