Rat GCK(Glucokinase) ELISA Kit

Rat

Hexokinase 4, FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2

F7EZK6

Sandwich

40ng/mL

0.38 ng/mL

0.63-40ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

CD & Adhesion molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GCK in the samples is then determined by comparing the OD of the samples to the standard curve.