Rat THP(Tamm–Horsfall Glycoprotein) ELISA Kit

Rat

Uromodulin, UMOD, ADMCKD2, FJHN, HNFJ, HNFJ1, MCKD2, THGP

P27590

Sandwich

200ng/mL

1.88 ng/mL

3.13-200ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to THP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain THP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THP in the samples is then determined by comparing the OD of the samples to the standard curve.