Rat CRT(Calreticulin) ELISA Kit

Rat

Calreticulin, calregulin,CRP55,CaBP3,calsequestrin-like protein, endoplasmic reticulum resident protein 6 ,ERp6,CALR, CRT, RO, SSA, cC1qR

P18418

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Metabolic pathway;Tumor immunity;Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CRT in the samples is then determined by comparing the OD of the samples to the standard curve.