Rat BRAK/CXCL14(Breast and Kidney Expressed Chemokine) ELISA Kit

Rat

CXCL14, SCYB14, NJAC, Bolekine, Kec, MIP-2G, BMAC, KS1

Sandwich

6000pg/mL

56.25 pg/mL

93.75-6000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BRAK/CXCL14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BRAK/CXCL14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BRAK/CXCL14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BRAK/CXCL14 in the samples is then determined by comparing the OD of the samples to the standard curve.