Rat AVPR1A(Arginine Vasopressin Receptor 1A) ELISA Kit

Rat

AVPR V1a, AVPR1, V1aR, V1a vasopressin receptor, antidiuretic hormone receptor 1A, SCCL vasopressin subtype 1a receptor, V1-vascular vasopressin receptor AVPR1A, vascular/hepatic-type arginine vasopressin receptor

P30560

Sandwich

30μg/mL

0.28 μg/mL

0.47-30μg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolism

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AVPR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AVPR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AVPR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AVPR1A in the samples is then determined by comparing the OD of the samples to the standard curve.