Rat TNFRSF1A(Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit

Rat

TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2, TNFR55, TNFR6, p55, p55-R, p6, CD12a, FPF, MS5, TBP1

P22934

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFRSF1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNFRSF1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFRSF1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNFRSF1A in the samples is then determined by comparing the OD of the samples to the standard curve.