Rat IL-12 p40(Interleukin 12 p40) ELISA Kit

Rat

IL12B, IL-12B, CLMF2, NKSF2, p4, IL-12/IL-23 p4

Q62712

Sandwich

800pg/mL

7.5 pg/mL

12.5-800pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Infection immunity;Neuro science;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-12 p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-12 p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-12 p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-12 p40 in the samples is then determined by comparing the OD of the samples to the standard curve.