高敏ELISA试剂盒

高敏小鼠白介素4(IL-4)酶联免疫吸附测定试剂盒
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货号

FY-HS-EM1391

样本类型

3.5H

检测时间

0.31-20pg/mL

价格(美元)

规格

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产品详情
英文名称:
High Sensitivity Mouse IL-4(Interleukin 4) ELISA Kit
种属:
FY-HS-EM1391-3
别称:
Mouse
Uniport ID:
B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4, IL-4B_cell stimulatory factor 1, IL4E12, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, pitrakinra
检测方法:
P07750
标准品:
Sandwich
灵敏度:
20pg/mL
检测范围:
0.19 pg/mL
反应时间:
0.31-20pg/mL
样品类型:
3.5H
研究领域:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=100μL
测试原理:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL-4 are added to the micro ELISA plate wells. Mouse IL-4 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL-4. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
20 2.347 2.301 2.324 2.284
10 1.606 1.454 1.53 1.49
5 0.917 0.881 0.899 0.859
2.5 0.424 0.408 0.416 0.376
1.25 0.232 0.242 0.237 0.197
0.63 0.133 0.141 0.137 0.097
0.32 0.092 0.088 0.09 0.05
0 0.042 0.038 0.04 0
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 85-110 93-112 92-105
Average (%) 100 110 93
1:4 Range (%) 90-115 88-107 93-111
Average (%) 115 107 110
1:8 Range (%) 90-100 88-110 90-107
Average (%) 91 91 102
1:16 Range (%) 93-100 93-111 93-106
Average (%) 99 94 95
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 88-111 101
EDTA plasma (n=8) 91-106 100
Cell culture media (n=8) 90-106 99
检测流程图
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