小鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒

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货号

FY-EM5060

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples

检测时间

3.5H

价格(美元)

280 400

规格

48T 96T

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DataSheet

说明书 MSDS

MSDS

产品详情性能参数操作步骤参考文献

产品详情

英文名称:

Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit

种属:

Mouse

别称:

HMGB1, HMG1, HMG3, SBP-1, Amphoterin

Uniport ID:

P63158

检测方法:

Sandwich

标准品:

1000pg/mL

灵敏度:

9.38 pg/mL

检测范围:

15.63-1000pg/mL

反应时间:

3.5H

样品类型:

Serum, Plasma, Tissue homogenate and Other biological samples

研究领域:

Cytokine;Infection immunity;

测试原理:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
1000	2.465	2.47	2.468	2.43
500	1.585	1.59	1.588	1.55
250	0.892	0.9	0.896	0.857
125	0.457	0.46	0.459	0.422
62.5	0.248	0.249	0.249	0.213
31.25	0.142	0.142	0.142	0.107
15.63	0.091	0.092	0.092	0.056
0	0.035	0.035	0.035	0

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	89-97	80-92	95-104
1:2	Average (%)	91	83	102
1:4	Range (%)	85-94	86-102	82-96
1:4	Average (%)	89	91	95
1:8	Range (%)	80-96	87-103	92-103
1:8	Average (%)	81	101	95
1:16	Range (%)	85-99	85-101	90-99
1:16	Average (%)	93	91	95

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	87-95	91
EDTA plasma (n=8)	96-107	102
Cell culture media (n=8)	80-96	87

检测流程图