Rat GTFⅡB(General Transcription Factor ⅡB) ELISA Kit

Rat

Transcription initiation factor IIB,Gtf2b,TF2B,General transcription factor TFIIB,RNA polymerase II alpha initiation factor

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GTF ⅡB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GTF ⅡB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GTF ⅡB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GTF ⅡB in the samples is then determined by comparing the OD of the samples to the standard curve.