Rat GCLM(Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit

Rat

GCS light chain,Gamma-ECS regulatory subunit,Gamma-glutamylcysteine synthetase regulatory subunit,Glutamate--cysteine ligase modifier subunit,GCLM, GLCLR

P48508

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCLM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GCLM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GCLM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GCLM in the samples is then determined by comparing the OD of the samples to the standard curve.