Human C-P(C-Peptide) ELISA Kit

Human

C-P; C-Peptide; IDDM2; ILPR; Insulin C-Peptide; insulin; IRDN; MODY10; Proinsulin C-Peptide; proinsulin

P01308

Sandwich

600pg/mL

5.62 pg/mL

9.38-600pg/mL

3.5H

Plasma；Sample Volume=100μL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-P. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-P in the samples is then determined by comparing the OD of the samples to the standard curve.