Human CA19-9(Carbohydrate antigen19-9) ELISA Kit

Human

sialylated Lewis(a) Antigen, Cancer Antigen 19-9

Sandwich

400IU/mL

3.75 IU/mL

6.25-400IU/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.