Human α2-M(Alpha-2 Macroglobulin) ELISA Kit

Human

α2M, A2M

P01023

Sandwich

4000ng/mL

37.5 ng/mL

62.5-4000ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

CD & Adhesion molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to α2-M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to α2-M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain α2-M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of α2-M in the samples is then determined by comparing the OD of the samples to the standard curve.