Human LL-37(Antibacterial Protein LL-37) ELISA Kit

Human

CAMP, CAP-18, CAP18, CRAMP, HSD26, LL37, cathelicidin antimicrobial peptide

P49913(134~170aa)

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Plasma

Signal transduction;Tumor immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.