英文名称:
Human USP8(Ubiquitin Specific Peptidase 8) ELISA Kit
别称:
Hum ORF8, SPG59, UBPY, PITA4, Ubiquitin carboxyl-terminal hydrolase 8,Deubiquitinating enzyme 8,Ubiquitin isopeptidase Y,hUBPy,Ubiquitin thioesterase 8,Ubiquitin-specific-processing protease 8,KIAA55
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples
研究领域:
Cell Biology;Cancer;Metabolism
测试原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to USP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to USP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain USP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of USP8 in the samples is then determined by comparing the OD of the samples to the standard curve.