Human TSP-1(Thrombospondin-1) ELISA Kit

Human

THBS1 , THBS-1, TSP, TSP1, Thrombospondin-1p18

P07996

Sandwich

500ng/mL

4.69 ng/mL

7.82-500ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

CD & Adhesion molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.