Human FcγR3A(Fc Fragment of IgG Low Affinity Ⅲa Receptor) ELISA Kit

Human

FCGR3A, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-1, FCRIII, FCRIIIA, IGFR3, IMD2, Fc fragment of IgG receptor IIIa

P08637

Sandwich

500ng/mL

4.69 ng/mL

7.82-500ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FcγR3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FcγR3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FcγR3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FcγR3A in the samples is then determined by comparing the OD of the samples to the standard curve.